Summary
Type: SPE
Application #: 303450
Catalog#: 730001 - SPE Cartridges, Chromabond, C18, 1ml, PP, 100mg, 100/pk
Detection: HPLC
Conditions: Column conditioning: The cartridges had been equilibrated with 10 ml of 40 mM choline in a methanol-acetonitrile-acetic acid (20:80:5, v/v/v).
Elution: Phosphatidylcholine was eluted using an additional 5 ml of 40 mM choline in a methanol-acetonitrile-acetic acid (20:80:5, v/v/v).
Evaporation: Eluates were collected in conical glass vials and evaporated to dryness at 50°C under a stream of nitrogen in a Thermovap evaporator. Residues were dissolved in 0,5 ml of ethanol.
Authors: Hradec J Dufek P
Source: Journal of Chromatograpy B 703 (1997) 259-262
Details
Legend: Recovery of added phosphatidylcholine:
Added (mg)
Recovery (%)
0,50
97,8
1,00
103,6
1,50
102,0
2,00
103,3
2,50
100,4